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Articles
Increasing the Efficiency of FLIPR
Utilization with a Modular Automated FLIPR Workstation
By Rebecca Hartlen
This application note describes how an Automated Fluorescence Imaging Plate Reader
(FLIPR) Workstation can increase FLIPR utilization and screening efficiency. Design emphasis for this workstation was on improving workflow around the FLIPR384 and on system modularity. The system was designed to automate agonist and antagonist assay protocols.
Identification of Glycosylated Peptides Using a Linear Ion Trap Mass Spectrometer
By Gargi Choudhary , Jae Schwartz , Diane Cho
Glycosylation is an important post-translational modification associated with many proteins that have a regulatory function. Several liquid chromatography and tandem mass spectrometry (LC–MS-MS) approaches have been used for analysis and structural elucidation of
glycoproteins. Most commonly, a glycoprotein is enzymatically digested, and the resulting fragments are fractionated by reversed-phase LC. The peptide fractions can be analyzed either by on-line MS-MS or collected and analyzed off-line by matrix-assisted laser desorption ionization
(MALDI) MS.
The Consequences of Limiting Stem Cell Research: Health and Economic Considerations
By Anthony J. Sinskey , Stan N. Finkelstein , Scott M. Cooper
In the 1913 edition, Webster's Dictionary defined scientific research as "diligent inquiry or examination in seeking facts or principles" — a definition more than suitable for today. Within the broad definition of scientific research, scientists generally acknowledge three types: basic, the primary purpose of which is to discover new facts or test theories about natural phenomena; experimental, in which conditions are varied to test effects and applied, where the investigation is conducted with a view to obtaining information directly useful in producing something with practical utility.
Microarray Data Comparability: An Array of Opportunity?
By Andrew I. Brooks
The landscape of high-throughput gene expression is forever changing and most recently has witnessed an onslaught of new microarray platforms aimed at enhancing the discovery process by improving on known problems with microarray technology. This begs the question, "Are new problems being generated with the advent of more specific and more sensitive arrays from new and existing commercial entities?"
High-throughput LC-based Assays for Screening of Caspase Inhibitors
By Jeff Koehler , Courtney Coyne
Separation-based assays performed using liquid chromatography (LC) offer several advantages when compared to assays performed using plate readers. LC-based assays reduce interference by separating quenchers and fluorescent compounds from the substrate. Also, simultaneous detection by ultraviolet (UV) absorbance and fluorescence provides additional assay information that is not available from a plate reader. While the throughput of conventional LC instrumentation limits its value for assay detection, micro parallel liquid chromatography (µPLC) increases analytical throughput by permitting analysis of 24 samples at the same time.
Simple Optimization of PCR Efficiency for Accurate Quantification of Gene Expression
By Haiying Grunenwald , Judith E. Meis , Katharine Kramer
Real-time RT-PCR is used extensively to quantify and validate gene expression analysis. PCR efficiency is a critical parameter for accurate quantification of gene expression, which can be optimized easily with
FailSafe" Real-Time PCR Optimization Kits.
Monolithic Nanocolumns in
LC–ESI-MS
By Jean-Pierre Chervet , Irina Dragan , Thomas Jakob , Remco Swart
Polymeric monolithic stationary phases offer an alternative to the classical microparticulate
sorbents, bringing important advantages to sample analysis. In contrast to the traditional stationary phases that consist of packed particles, the monolithic separation medium is made of a continuous, rigid polymeric rod with a porous structure.
High-throughput DNA Fragment Analysis with the cePRO 9600" System
By Wei Wei , Ho-Ming Pang , Jeremy Kenseth
The cePRO 9600" system from CombiSep provides high-throughput automated separation and quantitation of DNA fragments by 96-capillary array gel electrophoresis. On-line UV detection provides a sensitive, cost-effective means of analysis and eliminates the need for DNA labeling chemistries. The method provides superior separation resolution, improved quantitation and increased automation and throughput compared to agarose or polyacrylamide slab gels.
Homogenous
HitHunter? Assays for Kinase HTS
By Richard M. Eglen
The DiscoveRx HitHunter" technology provides a novel approach for kinase high-throughput screening. This homogeneous assay platform produces a positive chemiluminescent signal that is highly scalable to several microtiter plate formats and is markedly free from issues of optical interference. Collectively, the approach is readily configured for HTS assays at Ser, Thr and Tyr
kinases.
ProteinChip® Technology: Enabling the Discovery of Biomarker Assays for Drug Development
By Jennifer S. Cannon , Kate Gilbert
There is an increasing need among the translational medicine community for automated, quantitative protein biomarker assays that provide high predictive accuracy for disease diagnosis, prognosis and treatment response. Ciphergen"s ProteinChip® technology and Pattern Track" process were developed to facilitate the rapid translation of biomarker discoveries into validated assays.
Measuring
Allele-specific Expression Using MassARRAY®
By Chris Park , Devan Correll , Christian Jurinke , Paul Oeth
Allele-specific expression is an important link between individual genetic variation and disease (1, 2). Comparing allele-specific expression levels between groups, such as affected vs. unaffected, provides a basis for identifying disease susceptibility alleles and genes. To facilitate this process, quantitative methodologies for assessing allele-specific expression using the MassARRAY ® have been developed.
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