Articles
RNAi: A Robust Tool For Target
Identification And Validation
By Subrahmanyam Yerramilli , Eric Lader , Dirk Loeffert , Friederike
Wilmer , Peter Hahn , Elizabeth Scanlan
The application of RNA interference (RNAi) to mammalian cells has
significantly accelerated research in functional genomics and drug discovery.
RNAi allows simple, effective, and specific downregulation of mammalian gene
expression, making it a powerful and accessible technique with enormous
scientific, commercial, and potential therapeutic value.
In Silico Methods and Predictive Tools
Along the Drug Discovery Value Chain
By Dragan A. Cirovic , Vijay Bhargava , Frank Harrison , Roy Vaz , Abdel Laoui
The emerging drug discovery paradigm emphasizes the concept of in silico
methods for predicting in vitro and in vivo absorption, distribution,
metabolism, excretion and toxicological (ADMET) parameters. These methods are
being applied as early in the drug discovery value chain as possible to achieve
early attrition of unpromising candidate compounds.
"Moving Gene Expression Microarray Data to the Clinic – An Analytical
Requirements Perspective"
By Thomas Goralski
DNA microarrays are used to measure relative transcript abundance in RNA
samples, allowing researchers to identify differences in gene expression. To
date gene expression microarrays have predominantly served discovery based
research efforts, however, recent technological improvements and standardization
initiatives, as well as need driven incentives, have opened the possibility of
using this high-complexity analytical test in both pre-clinical and clinical
settings.
Magnetic
Beads Hold the Key to Novel Proteomic Analysis Using Mass Spectrometry
By Hege Skjellerudsveen
Key to successful scientific research in any discipline is obtaining
sufficient sample material. In particular for pharmaceutical research, protein
isolation procedures often need to produce samples that have high integrity and
concentration. Ideally, such a process would be easy to carry-out and, even more
importantly, produce the sample in a format that is readily applicable to the
experiment at hand.
A Cure for Multiple Sclerosis in Our Lifetime?
By Anthony J. Sinskey , Stan N. Finkelstein , Scott M. Cooper
Practice guidelines for MS in the United States and Canada recommend
disease-modifying agents for most patients, but at any given time only 20% are
using the drugs.
Interconnected Signaling Pathways Rethinking Drug Specificity as a Desirable
Objective
By Rangaprasad Sarangarajan , Shireesh Apte
Intracellular signaling occurs through cascades of
interconnected/interdependent networks, rather than through linear pathways.
Because effector molecules modulating this network typically function in both
redundant and pleiotypic fashion, selective modulation of a single intracellular
signaling pathway seems highly improbable.
